Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
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Validation data
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- Product number
- PAB7071 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7071, RRID:AB_1581852
- Product name
- VHL polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of VHL.
- Antigen sequence
C-RSLVKPENYRRLD
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Histone deacetylase inhibitors induce VHL and ubiquitin-independent proteasomal degradation of hypoxia-inducible factor 1alpha.
Kong X, Lin Z, Liang D, Fath D, Sang N, Caro J
Molecular and cellular biology 2006 Mar;26(6):2019-28
Molecular and cellular biology 2006 Mar;26(6):2019-28
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- VHL polyclonal antibody (Cat # PAB7071) (0.01 ug/mL) staining of human ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- VHL polyclonal antibody (Cat # PAB7071) (1.5 ug/mL) as the reporter as the capture rabbit antibody (2.5 ug/mL).
- Validation comment
- Sandwich ELISA