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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- TA327962 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal anti-SSRP1 Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal anti-SSRP1 Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SSRP1
- Isotype
- IgG
- Antibody clone number
- 30000
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- 293E cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-SSRP1 Antibody (clone 3E4). Proteins were visualized using a goat anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 ug/ml of purified anti-SSRP1 (clone 3E4) in blocking buffer overnight at 4°C, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue).
- Validation comment
- IF
