609709

antibody from BioLegend

Targeting: SSRP1 FACT80

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

609709

Provider

BioLegend

Proper citation

BioLegend Cat#609709, RRID:AB_2650946

Product name

Go-ChIP-Grade™ Purified anti-SSRP1

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

10D1

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3 µg of cross-linked chromatin samples from HeLa cells starved overnight and treated with 10% FCS with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-SSRP1 Antibody (Clone 10D1), B) equal amount of Purified Mouse IgG2b,k Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-SSRP1 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human EGR-1 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated