NB100-471
antibody from Novus Biologicals
Targeting: WRN
RECQ3, RECQL2
Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [4]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NB100-471 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-471, RRID:AB_2216077
- Product name
- Rabbit Polyclonal WRN Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Successful Cord Blood Transplantation in a Werner Syndrome Patient with High-risk Myelodysplastic Syndrome.
DNA2 drives processing and restart of reversed replication forks in human cells.
The Werner syndrome protein limits the error-prone 8-oxo-dG lesion bypass activity of human DNA polymerase kappa.
Identification and characterization of SMARCAL1 protein complexes.
Enhancement of human DNA polymerase η activity and fidelity is dependent upon a bipartite interaction with the Werner syndrome protein.
Human RECQ1 and RECQ4 helicases play distinct roles in DNA replication initiation.
Stabilization of quadruplex DNA perturbs telomere replication leading to the activation of an ATR-dependent ATM signaling pathway.
Cigarette smoke induces cellular senescence via Werner's syndrome protein down-regulation.
The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1.
Metal-catalyzed oxidation of the Werner syndrome protein causes loss of catalytic activities and impaired protein-protein interactions.
Hayashi K, Tasaka T, Kondo T, Ishikawa Y, Goto M, Matsuhashi Y, Sadahira Y, Sugihara T, Wada H
Internal medicine (Tokyo, Japan) 2019 Jan 1;58(1):109-113
Internal medicine (Tokyo, Japan) 2019 Jan 1;58(1):109-113
DNA2 drives processing and restart of reversed replication forks in human cells.
Thangavel S, Berti M, Levikova M, Pinto C, Gomathinayagam S, Vujanovic M, Zellweger R, Moore H, Lee EH, Hendrickson EA, Cejka P, Stewart S, Lopes M, Vindigni A
The Journal of cell biology 2015 Mar 2;208(5):545-62
The Journal of cell biology 2015 Mar 2;208(5):545-62
The Werner syndrome protein limits the error-prone 8-oxo-dG lesion bypass activity of human DNA polymerase kappa.
Maddukuri L, Ketkar A, Eddy S, Zafar MK, Eoff RL
Nucleic acids research 2014 Oct 29;42(19):12027-40
Nucleic acids research 2014 Oct 29;42(19):12027-40
Identification and characterization of SMARCAL1 protein complexes.
Bétous R, Glick GG, Zhao R, Cortez D
PloS one 2013;8(5):e63149
PloS one 2013;8(5):e63149
Enhancement of human DNA polymerase η activity and fidelity is dependent upon a bipartite interaction with the Werner syndrome protein.
Maddukuri L, Ketkar A, Eddy S, Zafar MK, Griffin WC, Eoff RL
The Journal of biological chemistry 2012 Dec 7;287(50):42312-23
The Journal of biological chemistry 2012 Dec 7;287(50):42312-23
Human RECQ1 and RECQ4 helicases play distinct roles in DNA replication initiation.
Thangavel S, Mendoza-Maldonado R, Tissino E, Sidorova JM, Yin J, Wang W, Monnat RJ Jr, Falaschi A, Vindigni A
Molecular and cellular biology 2010 Mar;30(6):1382-96
Molecular and cellular biology 2010 Mar;30(6):1382-96
Stabilization of quadruplex DNA perturbs telomere replication leading to the activation of an ATR-dependent ATM signaling pathway.
Rizzo A, Salvati E, Porru M, D'Angelo C, Stevens MF, D'Incalci M, Leonetti C, Gilson E, Zupi G, Biroccio A
Nucleic acids research 2009 Sep;37(16):5353-64
Nucleic acids research 2009 Sep;37(16):5353-64
Cigarette smoke induces cellular senescence via Werner's syndrome protein down-regulation.
Nyunoya T, Monick MM, Klingelhutz AL, Glaser H, Cagley JR, Brown CO, Matsumoto E, Aykin-Burns N, Spitz DR, Oshima J, Hunninghake GW
American journal of respiratory and critical care medicine 2009 Feb 15;179(4):279-87
American journal of respiratory and critical care medicine 2009 Feb 15;179(4):279-87
The human Werner syndrome protein stimulates repair of oxidative DNA base damage by the DNA glycosylase NEIL1.
Das A, Boldogh I, Lee JW, Harrigan JA, Hegde ML, Piotrowski J, de Souza Pinto N, Ramos W, Greenberg MM, Hazra TK, Mitra S, Bohr VA
The Journal of biological chemistry 2007 Sep 7;282(36):26591-602
The Journal of biological chemistry 2007 Sep 7;282(36):26591-602
Metal-catalyzed oxidation of the Werner syndrome protein causes loss of catalytic activities and impaired protein-protein interactions.
Harrigan JA, Piotrowski J, Di Noto L, Levine RL, Bohr VA
The Journal of biological chemistry 2007 Dec 14;282(50):36403-11
The Journal of biological chemistry 2007 Dec 14;282(50):36403-11
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WRN Antibody [NB100-471] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WRN Antibody [NB100-471] - Detection of WRN in 293T whole cell lysate using NB 100-471.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WRN Antibody [NB100-471] - Detection of human WRN on HeLa whole cell lysate using NB100-471. WRN was immunoprecipitated with NB100-471 or rabbit anti-WRN antibody NB100-472.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: WRN Antibody [NB100-471] - Human colon cancer cells with siRNA Control, siRNA pool of siWrn and 3 different sets of siRNA Wrn. GAPDH laoding control. Western blot image submitted by a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: WRN Antibody [NB100-471] - Detection of human WRN by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WRN antibody NB100-471 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. WRN was also immunoprecipitated by rabbit anti-WRN antibody NB100-472, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).