LS-C204591

antibody from LSBio

Targeting: UCHL1 PARK5, PGP9.5, Uch-L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C204591

Provider

LSBio

Product name

UCHL1 / PGP9.5 Antibody LS-C204591

Antibody type

Polyclonal

Description

Purified

Reactivity

Human

Host

Chicken/Avian

Isotype

IgY

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Blots of whole cell homogenate of the human SH-SY5Y neuroblastoma cell line stained with chicken antibody to glyceraldehyde 3 phosphate dehydrogenase (GAPDH, left lane, blot made with 1D4 monoclonal antibody to GAPDH) and UCHL1 / PGP9.5 antibody (right lane). The GAPDH antibody stains a ~37kDa band while the UCHL1 antibody stains a band at about 24kDa.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Rat mixed neuron/glial cultures stained with chicken UCHL1 (green) and rabbit antibody to glial fibrillary acidic protein (GFAP-red). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.