Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB12509 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB12509, RRID:AB_10678512
- Product name
- UCHL1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant UCHL1.
- Storage
- Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.
Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia.
The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.
Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr
Cell 2002 Oct 18;111(2):209-18
Cell 2002 Oct 18;111(2):209-18
Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia.
Kurihara LJ, Kikuchi T, Wada K, Tilghman SM
Human molecular genetics 2001 Sep 1;10(18):1963-70
Human molecular genetics 2001 Sep 1;10(18):1963-70
The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.
Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J
Science (New York, N.Y.) 1989 Nov 3;246(4930):670-3
Science (New York, N.Y.) 1989 Nov 3;246(4930):670-3
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of whole bovine brain extract stained with UCHL1 polyclonal antibody (Cat # PAB12509) showing a strong and clean band at about 24kDa.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Shows rat mixed neuron/glial cultures stained with UCHL1 polyclonal antibody (Cat # PAB12509) (green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.
- Validation comment
- Immunofluorescence