200-301-E52

antibody from Rockland Immunochemicals, Inc.

Targeting: UCHL1 PARK5, PGP9.5, Uch-L1

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

200-301-E52

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-301-E52, RRID:AB_11182569

Product name

Anti-Ubiquitin Hydrolase (UCHL1) (Mouse) Antibody - 200-301-E52

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. UCHL1 proteins are total IgG fractions. This antibody is directed against human UCHL1 protein. Reactivity is expected from the following species based on 100% sequence homology: most mammalian

Reactivity

Human, Rat, Bovine

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Storage

Store vial at -20 deg C prior to opening.Ê This product is stable at 4 deg C as an undiluted liquid.Ê For extended storage, aliquot contents and freeze at -20 deg C or below.Ê Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Handling

Dry Ice