Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NB600-1160 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-1160, RRID:AB_10000545
- Product name
- Mouse Monoclonal UCH-L1/PGP9.5 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified. This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C.
Submitted references Expression of Toll-like receptors 4 and 7 in murine peripheral nervous system development.
A light-gated potassium channel for sustained neuronal inhibition.
Pathologic Evaluation of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection at the Maternal-Fetal Interface of Late Gestation Pregnant Gilts.
Ubiquitin C-terminal hydrolase L1 deletion ameliorates glomerular injury in mice with ACTN4-associated focal segmental glomerulosclerosis.
Expression pattern and localization of alpha-synuclein in the human enteric nervous system.
Olmesartan ameliorates peripheral nerve dysfunction in Zucker diabetic fatty rats.
UCHL1 (PGP 9.5): neuronal biomarker and ubiquitin system protein.
Suitability of antigens PGP 9.5 and neurofilament light as marker proteins for detection of neuronal tissue in processed meat products.
Suitability of antigens PGP 9.5 and neurofilament light as marker proteins for detection of neuronal tissue in processed meat products.
Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization.
Distribution of cannabinoid receptor 1 (CB1) and 2 (CB2) on sensory nerve fibers and adnexal structures in human skin.
Arnaboldi F, Sommariva M, Opizzi E, Rasile M, Camelliti S, Busnelli M, Menegola E, Di Renzo F, Menon A, Barajon I
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2020 Sep;231:151526
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft 2020 Sep;231:151526
A light-gated potassium channel for sustained neuronal inhibition.
Alberio L, Locarno A, Saponaro A, Romano E, Bercier V, Albadri S, Simeoni F, Moleri S, Pelucchi S, Porro A, Marcello E, Barsotti N, Kukovetz K, Boender AJ, Contestabile A, Luo S, Moutal A, Ji Y, Romani G, Beltrame M, Del Bene F, Di Luca M, Khanna R, Colecraft HM, Pasqualetti M, Thiel G, Tonini R, Moroni A
Nature methods 2018 Nov;15(11):969-976
Nature methods 2018 Nov;15(11):969-976
Pathologic Evaluation of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection at the Maternal-Fetal Interface of Late Gestation Pregnant Gilts.
Novakovic P, Harding JC, Al-Dissi AN, Ladinig A, Detmer SE
PloS one 2016;11(3):e0151198
PloS one 2016;11(3):e0151198
Ubiquitin C-terminal hydrolase L1 deletion ameliorates glomerular injury in mice with ACTN4-associated focal segmental glomerulosclerosis.
Read NC, Gutsol A, Holterman CE, Carter A, Coulombe J, Gray DA, Kennedy CR
Biochimica et biophysica acta 2014 Jul;1842(7):1028-40
Biochimica et biophysica acta 2014 Jul;1842(7):1028-40
Expression pattern and localization of alpha-synuclein in the human enteric nervous system.
Böttner M, Zorenkov D, Hellwig I, Barrenschee M, Harde J, Fricke T, Deuschl G, Egberts JH, Becker T, Fritscher-Ravens A, Arlt A, Wedel T
Neurobiology of disease 2012 Dec;48(3):474-80
Neurobiology of disease 2012 Dec;48(3):474-80
Olmesartan ameliorates peripheral nerve dysfunction in Zucker diabetic fatty rats.
Sugimoto K, Kojima K, Baba M, Yasujima M
Journal of hypertension 2011 Jul;29(7):1337-46
Journal of hypertension 2011 Jul;29(7):1337-46
UCHL1 (PGP 9.5): neuronal biomarker and ubiquitin system protein.
Day IN, Thompson RJ
Progress in neurobiology 2010 Mar;90(3):327-62
Progress in neurobiology 2010 Mar;90(3):327-62
Suitability of antigens PGP 9.5 and neurofilament light as marker proteins for detection of neuronal tissue in processed meat products.
Gaunitz C, Gabert J, Lücker E, Seeger J, Stahl T
Journal of food protection 2009 May;72(5):1070-7
Journal of food protection 2009 May;72(5):1070-7
Suitability of antigens PGP 9.5 and neurofilament light as marker proteins for detection of neuronal tissue in processed meat products.
Gaunitz C, Gabert J, Lücker E, Seeger J, Stahl T
Journal of food protection 2009 May;72(5):1070-7
Journal of food protection 2009 May;72(5):1070-7
Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization.
Yi YJ, Manandhar G, Sutovsky M, Li R, Jonáková V, Oko R, Park CS, Prather RS, Sutovsky P
Biology of reproduction 2007 Nov;77(5):780-93
Biology of reproduction 2007 Nov;77(5):780-93
Distribution of cannabinoid receptor 1 (CB1) and 2 (CB2) on sensory nerve fibers and adnexal structures in human skin.
Ständer S, Schmelz M, Metze D, Luger T, Rukwied R
Journal of dermatological science 2005 Jun;38(3):177-88
Journal of dermatological science 2005 Jun;38(3):177-88
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Simple Western lane view shows a specific band for PGP9.5 / UCHL-1 in 0.2 mg/ml of h. Cerebellum (left) and IMR-32 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - analysis of UchL1 in 1) human, 2) mouse and 3) rat brain lysate using UchL1 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and ECL substrate solution were used for this test.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Electropherogram images of the corresponding Simple Western lane. PGP9.5 / UCHL-1 antibody was used at 10 ug/ml dilution of h. Cerebellum and IMR-32 lysates(s) respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Western Blot Analysis of human brain tissue lysate using UCH-L1/PGP9.5 Antibody (31A3)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Formalin-fixed, paraffin-embedded human brain stained with UchL1 antibody (PGP9.5) (1:500), peroxidase-conjugate and DAB chromogen. Staining seen in cytoplasm, ER and membrane. Fixation in 95% ethanol/5% acetic acid for 2-3 hours prior to paraffin embedding is recommended. Specimens which have not been fixed in acetic acid/alcohol require pre-treatment with citrate buffer: for example, unmask of the target epitope by boiling the tissue sections in 10mM citrate buffer, pH 6.0, for 10 min followed by cooling at room temp for 20 min.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: UCH-L1/PGP9.5 Antibody (31A3) [NB600-1160] - Flow Cytometric Analysis of T98G cells using UCH-L1/PGP9.5 Antibody (31A3)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).