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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- GTX81609 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX81609, RRID:AB_11167929
- Product name
- LIF antibody, Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Store at at 2-8¢XC for up to 6 months. For long term storage, aliquot and store at -20 ¢XC. Avoid repeated freeze/thaw cycles.
Submitted references Downregulation of genes related to immune and inflammatory response in IVF implantation failure cases under controlled ovarian stimulation.
Pathare ADS, Zaveri K, Hinduja I
American journal of reproductive immunology (New York, N.Y. : 1989) 2017 Jul;78(1)
American journal of reproductive immunology (New York, N.Y. : 1989) 2017 Jul;78(1)
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of LIF Antibody (Center) (GTX81609) in CEM cell line lysates (35μg/lane). LIF (arrow) was detected using the purified Pab.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of LIF (arrow) using rabbit polyclonal LIF Antibody (Center) (GTX81609). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LIF gene.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma reacted with LIF Antibody (Center)(GTX81609), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Flow cytometric analysis of CEM cells using LIF Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)(GTX81609). FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.
