LS-C742097

antibody from LSBio

Targeting: PRTN3 ACPA, AGP7, C-ANCA, MBT, P29, PR-3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C742097

Provider

LSBio

Product name

PRTN3 / Myeloblastin Antibody (clone 11F1A5) LS-C742097

Antibody type

Monoclonal

Description

Protein A affinity chromatography

Host

Mouse

Isotype

IgG

Antibody clone number

11F1A5

Storage

Long term: store at -20°C for up to 2 years. Reconstituted: store at 2°C to 8°C for up to 3 weeks or at -20°C for up to 1 year. Avoid freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Independent validation of Human PRTN3 Antibody (11F1A5) and Human PRTN3 Antibody (15E12D7) with Human PRTN3 recombinant protein. Lane 1: 50 ng Human PRTN3 recombinant protein Lane 2: 10 ng Human PRTN3 recombinant protein Lane 3: 5 ng Human PRTN3 recombinant protein Lane 4: 50 ng Human PRTN3 recombinant protein Lane 5: 10 ng Human PRTN3 recombinant protein Lane 6: 5 ng Human PRTN3 recombinant protein Primary Antibody: Lane 1~3: Human PRTN3 Antibody (11F1A5) 1 µg/ml Lane 4~6: Human PRTN3 Antibody (15E12D7) (H&L) [IRDye8°°] (Licor,926-32211)0

ELISA

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Standard curve of PRTN3 Sandwich ELISA. The PRTN3 Sandwich ELISA assay is developed by using Human PRTN3 Antibody (11F1A5) and Biotin conjugated Human PRTN3 Antibody (15E12D7) as capture and detect antibody, respectively. From the experimental results, Human PRTN3 Antibody (11F1A5) and Human PRTN3 Antibody (15E12D7) identify different epitopes. The sensitivity is <1 ng/ml and the detection range is 0-100 ng/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA binding of Human PRTN3 Antibody (11F1A5) with Human PRTN3 recombinant protein. Coating antigen: PRTN3, 1 µg/ml. PRTN3 antibody dilutions start from 1000 ng/ml, EC50= 9.667 ng/ml.