AF6134

antibody from R&D Systems

Targeting: PRTN3 ACPA, AGP7, C-ANCA, MBT, P29, PR-3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6134

Provider

R&D Systems

Product name

Human Proteinase 3/Myeloblastin/PRTN3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Proteinase 3/Myeloblastin/PRTN3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Cathepsin B and rhGranzyme B is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P24158


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Proteinase 3/Myeloblastin/ PRTN3 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, human peripheral blood lymphocytes (PBL), and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Proteinase 3/Myeloblastin/ PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Simple Western^TM. Simple Western lane view shows lysates of human peripheral blood mononuclear cell tissue, loaded at 0.2 mg/mL. A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.