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LearnPA5-92422
antibody from Invitrogen Antibodies
Targeting: APTX
AOA, AOA1, AXA1, EAOH, EOAHA, FLJ20157
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-92422 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Aprataxin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LYPYIVEFEE EAKNPGLETH RKRKRSGNSD SIERDAAQEA EAGTGLEPGS NSGQCSVPLK KGKDAPIKKE SLGHWSQGLK ISMQDPKMQV YKDEQVVVIK DKYPKARYHW LVLPWTSISS LKAVAREHLE LLKHMHTVGE KVIVDFAGSS KLRFRLGYHA IPSMSHVHLH VISQDFDSPC LKNKKHWNSF NTEYFLESQA VIEMVQEAGR VTVRDGMPEL LKLPLRCHEC QQLLPSIPQL KEHLRKHWTQ; Positive Samples: 22Rv1, A-549, K-562, BT-474, U-87MG, HT-29, Raji; Cellular Location: Cytoplasm, Nucleus, nucleolus, nucleoplasm
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.47 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Aprataxin in extracts of various cell lines using Aprataxin Polyclonal Antibody (Product # PA5-92422) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Aprataxin was performed in A549 cells using Aprataxin Polyclonal Antibody (Product # PA5-92422).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of Aprataxin was performed in 150 µg extracts of A549 cells using Aprataxin Polyclonal Antibody (Product # PA5-92422). Western blot was performed from the immunoprecipitate using Aprataxin Polyclonal Antibody at a dilution of 1:500.
