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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- APR-064-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Prostaglandin E Receptor EP2/PTGER2 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat brain (lanes 1 and 4), rat stomach (lanes 2 and 5) and mouse kidney (lanes 3 and 6) lysates: - 1-3. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:500).4-6. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody , preincubated with Prostaglandin E Receptor EP2/PTGER2 (extracellular) Blocking Peptide (#BLP-PR064).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human THP-1 acute monocytic leukemia cell lysates (lanes 1 and 4), human Jurkat T cell leukemia cell lysates (lanes 2 and 5) and human HT-29 colorectal adenocarcinoma cell lysates (lanes 3 and 6): - 1-3. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:500).4-6. Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody , preincubated with Prostaglandin E Receptor EP2/PTGER2 (extracellular) Blocking Peptide (#BLP-PR064).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression ofProstanoid EP2 receptor in rat cerebellum - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (1:100).Prostanoid EP2 receptor staining (red) is detected in Purkinje neurons and their dendritic tree (arrow). Blue is DAPI nissl counterstain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection of EP2 receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (2.5μg) + goat- Anti-rabbit-FITC.
