Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- A302-070A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RALY Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 0.20 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RALY by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RALY antibody (Product # A302-070A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 3 µg/mg lysate. RALY was successfully immunoprecipitated by rabbit anti-RALY antibody (Product # A302-069A), which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RALY by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-RALY antibody A302-070A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. RALY was successfully immunoprecipitated by rabbit anti-RALY antibody A302-069A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse RALY by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti- RALY (Cat. No. A302-070A Lot1) used at a dilution of 1:1,000 (0.2 µg/ml) and 1:200 (1µg/ml). Detection: DAB.