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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-21931 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PFKFB3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: A431, H1299.
- Concentration
- 0.95 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PFKFB3 using 30 µg of A) A431 and B) H1299 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PFKFB3 polyclonal antibody (Product # PA5-21931) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PFKFB3 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PFKFB3 Polyclonal Antibody (Product # PA5-21931) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PFKFB3 Polyclonal Antibody detects PFKFB3 protein at nucleus on BT483 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded BT483 xenograft. PFKFB3 Polyclonal Antibody (Product # PA5-21931) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of PFKFB3 was performed in A431 whole cell extracts using 5 µg of PFKFB3 Polyclonal Antibody (Product # PA5-21931). Samples were transferred to a membrane and probed with PFKFB3 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
