PAB20104

antibody from Abnova Corporation

Targeting: MBD4 MED1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB20104

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB20104, RRID:AB_10962260

Product name

MBD4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant MBD4.

Antigen sequence

LQNQSNNSNWNLRTRSKCKKDVFMPPSSSSELQES
RGLSNFTSTHLLLKEDEGVDDVNFRKVRKPKGKVT
ILKGIPIKKTKKGCRKSCSGFVQSDSKRESVCNKA
DAESEPVAQKSQLDRTVCISDAGACGETLSVTSEE
N


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with MBD4 polyclonal antibody (Cat # PAB20104) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with MBD4 polyclonal antibody (Cat # PAB20104) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human skin with MBD4 polyclonal antibody (Cat # PAB20104) shows strong nuclear positivity in epidermal cells and adnexal cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)