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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MAB2377 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal S100A10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse S100A10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A9, recombinant human (rh) S100B, or rhS100P is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references PSA-NCAM Colocalized with Cholecystokinin-Expressing Cells in the Hippocampus Is Involved in Mediating Antidepressant Efficacy.
Annexin2 coating the surface of enlargeosomes is needed for their regulated exocytosis.
Yamada J, Sato C, Konno K, Watanabe M, Jinno S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jan 22;40(4):825-842
The Journal of neuroscience : the official journal of the Society for Neuroscience 2020 Jan 22;40(4):825-842
Annexin2 coating the surface of enlargeosomes is needed for their regulated exocytosis.
Lorusso A, Covino C, Priori G, Bachi A, Meldolesi J, Chieregatti E
The EMBO journal 2006 Nov 29;25(23):5443-56
The EMBO journal 2006 Nov 29;25(23):5443-56
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse S100A10 by Western Blot. Western blot shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
