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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- AF2377 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse S100A10 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse S100A10 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human (rh) S100A10 is observed and less than 1% cross-reactivity with rhS100B and recombinant mouse S100A9 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P08207- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Ahnak scaffolds p11/Anxa2 complex and L-type voltage-gated calcium channel and modulates depressive behavior.
Gestational stress in mouse dams negatively affects gestation and postpartum hippocampal BDNF and P11 protein levels.
Molecular codes for cell type specification in Brn3 retinal ganglion cells.
Identification of the cortical neurons that mediate antidepressant responses.
Characterization of S100A11, a suppressive factor of fertilization, in the mouse female reproductive tract.
Role of p11 in cellular and behavioral effects of 5-HT4 receptor stimulation.
Jin J, Bhatti DL, Lee KW, Medrihan L, Cheng J, Wei J, Zhong P, Yan Z, Kooiker C, Song C, Ahn JH, Obermair GJ, Lee A, Gresack J, Greengard P, Kim Y
Molecular psychiatry 2020 May;25(5):1035-1049
Molecular psychiatry 2020 May;25(5):1035-1049
Gestational stress in mouse dams negatively affects gestation and postpartum hippocampal BDNF and P11 protein levels.
Vanmierlo T, De Vry J, Nelissen E, Sierksma A, Roumans N, Steinbusch HWM, Wennogle LP, van den Hove D, Prickaerts J
Molecular and cellular neurosciences 2018 Apr;88:292-299
Molecular and cellular neurosciences 2018 Apr;88:292-299
Molecular codes for cell type specification in Brn3 retinal ganglion cells.
Sajgo S, Ghinia MG, Brooks M, Kretschmer F, Chuang K, Hiriyanna S, Wu Z, Popescu O, Badea TC
Proceedings of the National Academy of Sciences of the United States of America 2017 May 16;114(20):E3974-E3983
Proceedings of the National Academy of Sciences of the United States of America 2017 May 16;114(20):E3974-E3983
Identification of the cortical neurons that mediate antidepressant responses.
Schmidt EF, Warner-Schmidt JL, Otopalik BG, Pickett SB, Greengard P, Heintz N
Cell 2012 May 25;149(5):1152-63
Cell 2012 May 25;149(5):1152-63
Characterization of S100A11, a suppressive factor of fertilization, in the mouse female reproductive tract.
Hanaue M, Miwa N, Uebi T, Fukuda Y, Katagiri Y, Takamatsu K
Molecular reproduction and development 2011 Feb;78(2):91-103
Molecular reproduction and development 2011 Feb;78(2):91-103
Role of p11 in cellular and behavioral effects of 5-HT4 receptor stimulation.
Warner-Schmidt JL, Flajolet M, Maller A, Chen EY, Qi H, Svenningsson P, Greengard P
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Feb 11;29(6):1937-46
The Journal of neuroscience : the official journal of the Society for Neuroscience 2009 Feb 11;29(6):1937-46
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse S100A10 by Western Blot. Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse S100A10 by Simple Western<SUP abp="264">TM. Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- S100A10 in Mouse Kidney. S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
