Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-82082 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- S100A10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MPSQMEHAME TMMFTFHKFA GDKGYLTKED LRVLMEKEFP GFLENQKDPL AVDKIMKDLD QCRDGKV
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Bu Shen Yi Sui Capsule Promotes Myelin Repair by Modulating the Transformation of A1/A2 Reactive Astrocytes In Vivo and In Vitro.
Zha Z, Liu YJ, Liu SS, Zhang N, Li JL, Qi F, Jin LY, Xue B, Yang T, Fan YP, Zhao H, Wang L
Oxidative medicine and cellular longevity 2022;2022:3800004
Oxidative medicine and cellular longevity 2022;2022:3800004
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S100A10 by a S100A10 polyclonal antibody (Product # PA5-82082). Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S100A10 by a S100A10 polyclonal antibody (Product # PA5-82082). Analysis in human cell lines Caco-2 and HEK293.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of S100A10 in U-2 OS cells using a S100A10 polyclonal antibody (Product # PA5-82082). The analysis shows localization to mitochondria.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S100A10 in human lung and skeletal muscle tissues using a S100A10 polyclonal antibody (Product # PA5-82082). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S100A10 in human lung using a S100A10 polyclonal antibody (Product # PA5-82082). The analysis shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S100A10 in human skeletal muscle using a S100A10 polyclonal antibody (Product # PA5-82082). The analysis shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 8 BSYS modulates the A1/A2 Astrocytes transformation by suppressing the miR-155 levels in vitro. (a) Immunocytofluorescence staining of astrocytes per group utilizing antibodies specific for GFAP (red), C3 (green), and S100A10 (green). Scale bars: 50 mu m. (b, c) Measurement of the proportion of C3+/GFAP+ and S100A10+/GFAP+ cells in astrocytes. (d) Illustrative Western blotting images and quantitative data of C3 and S100A10 in astrocytes. Data are reported as means +- SD; as opposed to the CON group, ### P < 0.001; versus the ASC group, * P < 0.05, ** P < 0.01, and *** P < 0.001; contrasted with the ASC+blank serum group, *** P < 0.001; vs. the ASC+BSYS group, ^^^ P < 0.001; vs. the ASC+BSYS+miR-NC group, ^^^ P < 0.001.