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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00005026-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005026-M01, RRID:AB_546715
- Product name
- Mouse Monoclonal P2X5/P2RX5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. P2RX5 - purinergic receptor P2X, ligand-gated ion channel, 5
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references A truncation variant of the cation channel P2RX5 is upregulated during T cell activation.
Abramowski P, Ogrodowczyk C, Martin R, Pongs O
PloS one 2014;9(9):e104692
PloS one 2014;9(9):e104692
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Analysis of P2RX5 over-expressed 293 cell line, cotransfected with P2RX5 Validated Chimera RNAi ( Cat # H00005026-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with P2RX5 monoclonal antibody (M01), clone 1C5 (Cat # H00005026-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Analysis of P2RX5 expression in transfected 293T cell line by P2RX5 monoclonal antibody (M01), clone 1C5.Lane 1: P2RX5 transfected lysate(46.42 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: P2X5/P2RX5 Antibody (1C5) [H00005026-M01] - Detection limit for recombinant GST tagged P2RX5 is approximately 1ng/ml as a capture antibody.
