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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX113821 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113821, RRID:AB_2037840
- Product name
- Rap1GAP antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 (GTX27909) 7.5% SDS PAGE GTX113821 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rap1GAP antibody [N2C2], Internal (GTX113821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rap1GAP antibody [N2C2], Internal detects Rap1GAP protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Rap1GAP antibody [N2C2], Internal (GTX113821) diluted at 1:500.
