NBP1-33710

antibody from Novus Biologicals

Targeting: RAP1GAP KIAA0474, RAP1GA1, RAP1GAP1, RAP1GAPII

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-33710

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-33710, RRID:AB_2177288

Product name

Rabbit Polyclonal RAP1GAP Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RAP1GAP Antibody [NBP1-33710] - Sample (30 ug of whole cell lysate) A: A431 7. 5% SDS PAGE, antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RAP1GAP Antibody [NBP1-33710] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Rap1GAP antibody [N2C2], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RAP1GAP Antibody [NBP1-33710] - Rap1GAP antibody [N2C2], Internal detects Rap1GAP protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Rap1GAP antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min