Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX124186 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX124186, RRID:AB_11169084
- Product name
- CHD4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references CHD4-mediated loss of E-cadherin determines metastatic ability in triple-negative breast cancer cells.
The NuRD complex-mediated p21 suppression facilitates chemoresistance in BRCA-proficient breast cancer.
Senataxin controls meiotic silencing through ATR activation and chromatin remodeling.
Luo CW, Wu CC, Chang SJ, Chang TM, Chen TY, Chai CY, Chang CL, Hou MF, Pan MR
Experimental cell research 2018 Feb 1;363(1):65-72
Experimental cell research 2018 Feb 1;363(1):65-72
The NuRD complex-mediated p21 suppression facilitates chemoresistance in BRCA-proficient breast cancer.
Hou MF, Luo CW, Chang TM, Hung WC, Chen TY, Tsai YL, Chai CY, Pan MR
Experimental cell research 2017 Oct 15;359(2):458-465
Experimental cell research 2017 Oct 15;359(2):458-465
Senataxin controls meiotic silencing through ATR activation and chromatin remodeling.
Yeo AJ, Becherel OJ, Luff JE, Graham ME, Richard D, Lavin MF
Cell discovery 2015;1:15025
Cell discovery 2015;1:15025
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 5% SDS PAGE GTX124186 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CHD4(GTX124186) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 £gg of CHD4 antibody (GTX124186).Western blot analysis was performed using CHD4 antibody (GTX124186).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of CHD4 protein from 293T whole cell extracts using 5 £gg of CHD4 antibody (GTX124186) or CHD4 antibody (GTX101662).Western blot analysis was performed using CHD4 antibody (GTX124186).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded D54 xenograft, using CHD4(GTX124186) antibody at 1:1000 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CHD4 antibody detects CHD4 protein at cytosol and nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. CHD4 antibody (GTX124186) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CHD4 antibody detects CHD4 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. CHD4 antibody (GTX124186) dilution: 1:500.
