GTX70019

antibody from GeneTex

Targeting: NCOR2 CTG26, SMRT, SMRTE, TNRC14, TRAC-1

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX70019

Provider

GeneTex

Proper citation

GeneTex Cat#GTX70019, RRID:AB_11165050

Product name

SMRT antibody [1212]

Antibody type

Monoclonal

Host

Mouse

Vial size

100 µg

References

Submitted references

Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells.

Cassidy L, Choi M, Meyer J, Chang R, Seigel GM
Journal of biomarkers 2013;2013:960862

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An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells.

Tang C, Lee AS, Volkmer JP, Sahoo D, Nag D, Mosley AR, Inlay MA, Ardehali R, Chavez SL, Pera RR, Behr B, Wu JC, Weissman IL, Drukker M
Nature biotechnology 2011 Aug 14;29(9):829-34

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% FA fixed human embryonic stem cells H9 using anti-SSEA-5 Ab (1:50 dilution). (A) Brightfield image(B) Dapi(C) anti-SSEA-5 Ab(D) Merged image of anti-SSEA-5 Ab and DAPI

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% PFA fixed human embryonal carcinoma cell line Tera using anti-SSEA-5 Ab (1:1000 dilution). SSEA-5 positive cells (red) represented pluripotent population; negative cells represented differentiated population. DAPI (Blue) indicated nuclear staining.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Pluripotent stem cell population indicated by SSEA5-staining. Human embryonic stem cells H9 were analyzed with SSEA-5 mAB (pseudo-colored in red) and control IgG (pseudo-colored in green) in flow cytometry. Each Ab was used at a 1:50 dilution in PBS.