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Western blot
Immunoprecipitation
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-46939 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Trypsin Pan Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Trypsin Pan in recombinant human Trypsin 1, 2, and 3. Samples were incubated in Trypsin Pan polyclonal antibody (Product # PA5-46939) using a dilution of 1 µg/mL followed by HRP-conjugated Anti-Sheep IgG. A specific band was detected for Trypsin at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Trypsin Pan in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue. Samples were incubated in Trypsin Pan polyclonal antibody (Product # PA5-46939) using a dilution of 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cancer cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Trypsin Pan in immersion fixed paraffin-embedded sections of human pancreas. Samples were incubated in Trypsin Pan polyclonal antibody (Product # PA5-46939) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Trypsin Pan was performed using human pancreas. Antigen-antibody complexes were formed by incubating 500 μg human pancreas lysates with Trypsin Pan polyclonal antibody (Product # PA5-46939) using a dilution of 12.5 µg. The Trypsin 2/PRSS2-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated human Trypsin 2/PRSS2 was detected by Western blot using 2 µg/mL of Sheep Anti-Human Trypsin Pan Specific (PRSS1/2/3) Antigen Affinity-purified Polyclonal Antibody under non-reducing conditions.
