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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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Validation data
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- Product number
- PA1-4515 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PINK1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-4515 detects PINK1 in human samples. PA1-4515 has been successfully used in Western blotting procedures. By Western blot, this antibody detects a ~64 kDa protein representing PINK1 in transfected HEK293T cells. PA1-4515 immunizing peptide corresponds to amino acid residues 258-274 of human PINK1.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Mutated FANCA Gene Role in the Modulation of Energy Metabolism and Mitochondrial Dynamics in Head and Neck Squamous Cell Carcinoma.
Bertola N, Degan P, Cappelli E, Ravera S
Cells 2022 Jul 30;11(15)
Cells 2022 Jul 30;11(15)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of PINK1. Lane 1 shows V5His-tagged PINK1 protein and lane 2 shows myc-tagged PINK1 protein in HEK293T cells using Product # PA1-4515.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression evaluation of proteins involved in mitophagy and autophagy processes in OHSU-974-FAcorr and OHSU-974-S91. ( A ) Western blot signals of Pink1 and Parkin (two mitophagy markers), Beclin1, Atg7, Atg12, Atg16L1, LC3 (autophagy markers), and beta-Actin. ( B ) Densitometric analysis of WB signals reported in Panel A, normalized versus beta-Actin. Data in histograms are reported as mean +- SD and are representative of at least 3 independent experiments. Statistical significance was tested with an unpaired t -test. * represents a p < 0.05 between OHSU-974-S91 cells and the OHSU-974-FAcorr cells used as control.
