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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- 14782-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SMTNL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MRKTSNSCI MENGHQPGTG PGDGPPEIAQ NFSAPDPPRP RPVSLSLRLP HQPVTAITRV SDRFSGETSA AALSPMSAAT LGGLNPSPSE VITPWTPSPS EKNSSFTWSV PSSGYGAVTA SKHSNSPPLV TPPQSPVSPQ PPAITQVHRQ GERRRELVRS QTLPRTSEAQ ARKALFEKWE QETAAGKGKG EARARLKRSQ SFGVASASSI KQILLEWCRS KTLGYQHVDL QNFSSSWSDG MAFCALVHSF FPDAFDYNSL SPTQRQKNFE LAFTMAENLA NCERLIEVED MMVMGRKPDP MCVFTYVQSL YNHLRRFE (1-317 aa encoded by BC112177)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SGC-7901 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed MCF-7 cells using 14782-1-AP (SMTNL2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-SMTNL2 (IP:14782-1-AP, 4ug; Detection:14782-1-AP 1:500) with COLO 320 cells lysate 4000ug.
