MAB8075

antibody from R&D Systems

Targeting: SATB1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB8075

Provider

R&D Systems

Product name

Human/Mouse SATB1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse SATB1 in ELISAs and Western blots.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q01826


Isotype

IgG

Antibody clone number

815427

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SATB1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human SATB1 Monoclonal Antibody (Catalog # MAB8075) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SATB1 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human SATB1 by Simple Western^TM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for SATB1 at approximately 113 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse SATB1 Monoclonal Antibody (Catalog # MAB8075) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.