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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX114738 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX114738, RRID:AB_11167788
- Product name
- SATB1 antibody [C2C3], C-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references SATB1 promotes tumor metastasis and invasiveness in oral squamous cell carcinoma.
Li YC, Bu LL, Mao L, Ma SR, Liu JF, Yu GT, Deng WW, Zhang WF, Sun ZJ
Oral diseases 2017 Mar;23(2):247-254
Oral diseases 2017 Mar;23(2):247-254
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114738) diluted by 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SATB1 antibody detects SATB1 protein by western blot analysis. Whole cell extracts (30 £gg) was separated by 7.5 % SDS-PAGE, and blotted with SATB1 antibody (GTX114738) diluted by 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 antibody [C2C3], C-term (GTX114738) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- SATB1 antibody immunoprecipitates SATB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 £gg of preimmune rabbit IgG B. Immunoprecipitation of SATB1 protein by 2 £gg of SATB1 antibody (GTX114738) 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody (GTX114738) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SATB1(GTX114738) antibody at 1:250 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with Jurkat chromatin extract and 5 £gg of either control rabbit IgG or anti-SATB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.
