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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Chromatin Immunoprecipitation [1]
- Other assay [2]
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- Product number
- PA5-30163 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SATB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, THP-1, mouse thymus.
- Concentration
- 0.36 mg/mL
Submitted references Inhibition of prostate cancer DU145 cell growth with small interfering RNA targeting the SATB1 gene.
Wang Q, Yang CS, Ma ZX, Chen JC, Zheng JN, Sun XQ, Wang JQ
Experimental and therapeutic medicine 2018 Mar;15(3):3028-3033
Experimental and therapeutic medicine 2018 Mar;15(3):3028-3033
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SATB1 using Whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SATB1 polyclonal antibody (Product # PA5-30163) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SATB1 using Whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a SATB1 polyclonal antibody (Product # PA5-30163) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SATB1 was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a SATB1 Polyclonal Antibody (Product # PA5-30163) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SATB1 Polyclonal Antibody (Product # PA5-30163). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SATB1 Polyclonal Antibody (Product # PA5-30163) diluted at 1:40,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SATB1 Polyclonal Antibody (Product # PA5-30163) and a ~100 kDa band corresponding to SATB1 was observed in across the tissues tested except Mouse Kidney which is reported to be negative. An uncharacterized band (*) at ~60 kDa was observed in Mouse Thymus. Tissue extracts (30ug lysate) of Mouse Spleen (Lane 1), Mouse Thymus (Lane 2) and Mouse Kidney (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SATB1 Polyclonal Antibody detects SATB1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SATB1 stained by SATB1 Polyclonal Antibody (Product # PA5-30163) diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SATB1 (Product # PA5-30163) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SATB1 Polyclonal Antibody detects SATB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. SATB1 stained by SATB1 Polyclonal Antibody (Product # PA5-30163) diluted at 1:500. Antigen Retrieval: Citrate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SATB1 Polyclonal Antibody detects SATB1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. SATB1 stained by SATB1 Polyclonal Antibody (Product # PA5-30163) diluted at 1:500. Antigen Retrieval: Citra.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with Jurkat chromatin extract and 5 µg of either control rabbit IgG or SATB1 Polyclonal Antibody (Product # PA5-30163). The precipitated DNA was detected by PCR with primer set targeting to BCL2 promoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SATB1 antibody immunoprecipitates SATB1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of SATB1 protein by 2 µg of SATB1 antibody (Product # PA5-30163) 7.5% SDS-PAGE The immunoprecipitated SATB1 protein was detected by SATB1 antibody (Product # PA5-30163) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3. Protein expression levels of SATB1. (A) Western blot analysis was performed to determine SATB1 protein expression levels following siRNA2 transfection compared with the NC and Blank groups (n=5). (B) ImageJ quantitation of the band densities from the western blot analysis of SATB1 was performed. *P
