PAB18217

antibody from Abnova Corporation

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB18217

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB18217, RRID:AB_10716481

Product name

RAF1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of RAF1.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Protein kinase C and epidermal growth factor stimulation of Raf1 potentiates adenylyl cyclase type 6 activation in intact cells.

Beazely MA, Alan JK, Watts VJ
Molecular pharmacology 2005 Jan;67(1):250-9

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Positive regulation of Raf1-MEK1/2-ERK1/2 signaling by protein serine/threonine phosphatase 2A holoenzymes.

Adams DG, Coffee RL Jr, Zhang H, Pelech S, Strack S, Wadzinski BE
The Journal of biological chemistry 2005 Dec 30;280(52):42644-54

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of extracts from HeLa cells, treated with UV (5 mins), using RAF1 polyclonal antibody (Cat # PAB18217).Peptide "+" means "peptide blocking".

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells, using RAF1 polyclonal antibody (Cat # PAB18217).Peptide "+" means "peptide blocking".

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using RAF1 polyclonal antibody (Cat # PAB18217).Peptide "+" means "peptide blocking".

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)