PA5-17699

antibody from Invitrogen Antibodies

Targeting: IGFBP2 IBP2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA5-17699

Provider

Invitrogen Antibodies

Product name

IGFBP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with other IGFBP family members.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

5 µg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-IGFBP2 Polyclonal Antibody, (Product # PA5-17699) and a 35 kDa band corresponding to IGFBP2 was observed in the cell lines tested and increased upon protein transport inhibitor in SH-SY5Y. Membrane enriched cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), SH-SY5Y treated with Protein Transport Inhibitor (1X for 4 hours) (Lane 2), OVCAR-3 (Lane 3), and SK-OV-3 (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).