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antibody from Invitrogen Antibodies
Targeting: NR1D2
BD73, EAR-1r, Hs.37288, HZF2, REVERBB, REVERBbeta, RVR
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-80866 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-NR1D2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human NR1D2.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 5 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NR1D2 in Lane A: HeLa Whole Cell Lysate (30 µg), Lane B: Jurkat Whole Cell Lysate (30 µg), Lane C: THP1 Whole Cell Lysate (30 µg). Samples were probed using a NR1D2 Polyclonal Antibody (Product # PA5-80866) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:65 kDa. Observed band size:55 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NR1D2 Polyclonal Antibody (Product # PA5-80866) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: Jurkat Whole Cell Lysate, Lane C: THP1 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti- RabbitIgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 65 kDa. Observed band size: 55 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human NR1D2 in human brain with NR1D2 Polyclonal Antibody (Product # PA5-80866, 1:100,000, formalin-fixed paraffin embedded sections).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NR1D2 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate, Lane B: 0.5 mg Hela Whole Cell Lysate 0.5 µL with NR1D2 Polyclonal Antibody (Product # PA5-80866) and 15 µL of 50 % Protein G agarose. Primary antibody: NR1D2 Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 63 kDa. Observed band size: 55 kDa.
