PAB3478

antibody from Abnova Corporation

Targeting: ARAF ARAF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3478

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3478, RRID:AB_1571316

Product name

ARAF polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of ARAF.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Selective activation of MEK1 but not MEK2 by A-Raf from epidermal growth factor-stimulated Hela cells.

Wu X, Noh SJ, Zhou G, Dixon JE, Guan KL
The Journal of biological chemistry 1996 Feb 9;271(6):3265-71

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The complete sequence and promoter activity of the human A-raf-1 gene (ARAF1).

Lee JE, Beck TW, Brennscheidt U, DeGennaro LJ, Rapp UR
Genomics 1994 Mar 1;20(1):43-55

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Localization of the pKs gene, a raf related sequence on human chromosomes X and 7.

Popescu NC, Mark GE
Oncogene 1989 Apr;4(4):517-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of MCF-7 cell lysate (35 ug/lane) with ARAF polyclonal antibody (Cat # PAB3478).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with ARAF polyclonal antibody (Cat # PAB3478) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)