HPA023818

antibody from Atlas Antibodies

Targeting: DNAJB5 Hsc40

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA023818

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA023818, RRID:AB_1847793

Product name

Anti-DNAJB5

Antibody type

Polyclonal

Description

Affinity purified using the PrEST antigen as affinity ligand

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

FHYTFHGDPHATFASFFGGSNPFDIFFASSRSTRP
FSGFDPDDMDVDEDEDPFGAFGRFGFNGLSRGPRR
APEPLYPRRKVQDPPVVHELRVSLEEIYHGSTK


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and DNAJB5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415870).

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human smooth muscle and liver tissues using HPA023818 antibody. Corresponding DNAJB5 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in glial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human smooth muscle shows moderate nuclear positivity.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows moderate nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows very weak positivity in hepatocytes.

Sample type

HUMAN