Antibody data
- Antibody Data
- Antigen structure
- References [13]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB970-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TIMP-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human TIMP-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse TIMP-1 or recombinant human TIMP-2.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01033
- Isotype
- IgG
- Antibody clone number
- 63515
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Tissue Inhibitor of Metalloproteinase-1 Promotes Polymorphonuclear Neutrophil (PMN) Pericellular Proteolysis by Anchoring Matrix Metalloproteinase-8 and -9 to PMN Surfaces.
Increased expression of matrix metalloproteinase-1 in systemic vessels of preeclamptic women: a critical mediator of vascular dysfunction.
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
A multiplex immunoassay for human adipokine profiling.
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Matrix metalloproteinase activity in pediatric acute lung injury.
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Mediators of glioblastoma resistance and invasion during antivascular endothelial growth factor therapy.
Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Limited processing of pro-matrix metalloprotease-2 (gelatinase A) overexpressed by transfection in PC-3 human prostate tumor cells: association with restricted cell surface localization of membrane-type matrix metalloproteinase-1.
Wang X, Rojas-Quintero J, Wilder J, Tesfaigzi Y, Zhang D, Owen CA
Journal of immunology (Baltimore, Md. : 1950) 2019 Jun 1;202(11):3267-3281
Journal of immunology (Baltimore, Md. : 1950) 2019 Jun 1;202(11):3267-3281
Increased expression of matrix metalloproteinase-1 in systemic vessels of preeclamptic women: a critical mediator of vascular dysfunction.
Estrada-Gutierrez G, Cappello RE, Mishra N, Romero R, Strauss JF 3rd, Walsh SW
The American journal of pathology 2011 Jan;178(1):451-60
The American journal of pathology 2011 Jan;178(1):451-60
The DSCs-expressed CD82 controls the invasiveness of trophoblast cells via integrinbeta1/MAPK/MAPK3/1 signaling pathway in human first-trimester pregnancy.
Li MQ, Hou XF, Shao J, Tang CL, Li DJ
Biology of reproduction 2010 May;82(5):968-79
Biology of reproduction 2010 May;82(5):968-79
A multiplex immunoassay for human adipokine profiling.
Schipper HS, de Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clinical chemistry 2010 Aug;56(8):1320-8
Clinical chemistry 2010 Aug;56(8):1320-8
Chondrocytes from patients with osteoarthritis express typical extracellular matrix molecules once grown onto a three-dimensional hyaluronan-based scaffold.
Cavallo C, Desando G, Facchini A, Grigolo B
Journal of biomedical materials research. Part A 2010 Apr;93(1):86-95
Journal of biomedical materials research. Part A 2010 Apr;93(1):86-95
Matrix metalloproteinase activity in pediatric acute lung injury.
Kong MY, Gaggar A, Li Y, Winkler M, Blalock JE, Clancy JP
International journal of medical sciences 2009;6(1):9-17
International journal of medical sciences 2009;6(1):9-17
Tissue inhibitors of matrix metalloproteinases in platelets and megakaryocytes: a novel organization for these secreted proteins.
Villeneuve J, Block A, Le Bousse-Kerdilès MC, Lepreux S, Nurden P, Ripoche J, Nurden AT
Experimental hematology 2009 Jul;37(7):849-56
Experimental hematology 2009 Jul;37(7):849-56
Mediators of glioblastoma resistance and invasion during antivascular endothelial growth factor therapy.
Lucio-Eterovic AK, Piao Y, de Groot JF
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jul 15;15(14):4589-99
Clinical cancer research : an official journal of the American Association for Cancer Research 2009 Jul 15;15(14):4589-99
Use of an immunoaffinity-mass spectrometry-based approach for the quantification of protein biomarkers from serum samples of lung cancer patients.
Nicol GR, Han M, Kim J, Birse CE, Brand E, Nguyen A, Mesri M, FitzHugh W, Kaminker P, Moore PA, Ruben SM, He T
Molecular & cellular proteomics : MCP 2008 Oct;7(10):1974-82
Molecular & cellular proteomics : MCP 2008 Oct;7(10):1974-82
Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
Naveau A, Reinald N, Fournier B, Durand E, Lafont A, Coulomb B, Gogly B
Connective tissue research 2007;48(6):300-8
Connective tissue research 2007;48(6):300-8
IL-21 is highly produced in Helicobacter pylori-infected gastric mucosa and promotes gelatinases synthesis.
Caruso R, Fina D, Peluso I, Fantini MC, Tosti C, Del Vecchio Blanco G, Paoluzi OA, Caprioli F, Andrei F, Stolfi C, Romano M, Ricci V, MacDonald TT, Pallone F, Monteleone G
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
Journal of immunology (Baltimore, Md. : 1950) 2007 May 1;178(9):5957-65
Control of matrix metalloproteinase production in human intestinal fibroblasts by interleukin 21.
Monteleone G, Caruso R, Fina D, Peluso I, Gioia V, Stolfi C, Fantini MC, Caprioli F, Tersigni R, Alessandroni L, MacDonald TT, Pallone F
Gut 2006 Dec;55(12):1774-80
Gut 2006 Dec;55(12):1774-80
Limited processing of pro-matrix metalloprotease-2 (gelatinase A) overexpressed by transfection in PC-3 human prostate tumor cells: association with restricted cell surface localization of membrane-type matrix metalloproteinase-1.
Wilson MJ, Jiang A, Wiehr C, Wang X, Sinha AA, Pei D
Journal of andrology 2004 Mar-Apr;25(2):274-85
Journal of andrology 2004 Mar-Apr;25(2):274-85
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TIMP-1 in Wi-38 Human Cell Line. TIMP-1 was detected in immersion fixed Wi-38 human lung fibroblast cell line using Mouse Anti-Human TIMP-1 Monoclonal Antibody (Catalog # MAB970) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TIMP-1 in Human Astrocytoma. TIMP-1 was detected in immersion fixed paraffin-embedded sections of human astrocytoma using Mouse Anti-Human TIMP-1 Monoclonal Antibody (Catalog # MAB970) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.