Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00007076-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00007076-M01, RRID:AB_530221
- Product name
- TIMP1 monoclonal antibody (M01), clone 4D12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant TIMP1.
- Antigen sequence
MAPFEPLASGILLLLWLIAPSRACTCVPPHPQTAF
CNSDLVIRAKFVGTPEVNQTTLYQRYEIKMTKMYK
GFQALGDAADIRFVYTPAMESVCGYFHRSHNRSEE
FLIAGKLQDGLLHITTCSFVAPWNSLSLAQRRGFT
KTYTVGCEECTVFPCLSIPCKLQSGTHCL- Isotype
- IgG
- Antibody clone number
- 4D12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TIMP1 expression in transfected 293T cell line by TIMP1 monoclonal antibody (M01), clone 4D12.Lane 1: TIMP1 transfected lysate(23.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged TIMP1 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of TIMP1 transfected lysate using anti-TIMP1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with TIMP1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
