NBP1-49973

antibody from Novus Biologicals

Targeting: RNGTT hCAP, HCE, HCE1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-49973

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-49973, RRID:AB_10012163

Product name

Rabbit Polyclonal RNGTT Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

The cytoplasmic capping complex assembles on adapter protein nck1 bound to the proline-rich C-terminus of Mammalian capping enzyme.

Mukherjee C, Bakthavachalu B, Schoenberg DR
PLoS biology 2014 Aug;12(8):e1001933

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: RNGTT Antibody [NBP1-49973] - Detection of Human and Mouse RNGTT by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-RNGTT antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. RNGTT was also immunoprecipitated by rabbit anti-RNGTT antibody which recognizes an upstream epitope. For blotting immunoprecipitated RNGTT was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: RNGTT Antibody [NBP1-49973] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RNGTT used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate.