MA1-19285

antibody from Invitrogen Antibodies

Targeting: SIT1 SIT

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-19285

Provider

Invitrogen Antibodies

Product name

SIT Monoclonal Antibody (SIT-01)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

Western Blot: Reducing conditions.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

SIT-01

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C, do not freeze

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-SIT Monoclonal antibody (Product # MA1-192850) followed by IRDye800-conjugated anti-mouse secondary antibody. SIT was detected around 36kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blotting analysis of human SIT using mouse monoclonal antibody SIT-01 on lysates of Molt-4 and HEK-293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-SIT Monoclonal antibody (Product # MA1-192850) followed by IRDye800-conjugated anti-mouse secondary antibody. SIT was detected around 36kDa.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Separation of human CD3 negative SIT positive lymphocytes (red-filled) from CD3 negative SIT negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral human whole blood stained using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-SIT (SIT-01) purified Monoclonal antibody (Product # MA1-19285) (concentration in sample 9 µg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue™ using a dilution of 20 µL reagent/100 µL of peripheral whole blood.