PA3-028

antibody from Invitrogen Antibodies

Targeting: OPRK1 KOR, OPRK

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA3-028

Provider

Invitrogen Antibodies

Product name

OPRK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

IHC (P) analysis shows positive staining of KOR in human brain.

Concentration

Conc. Not Determined

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KOR was performed by loading equal amounts of wheat germ lectin agarose bead enriched KOR receptor fractions from mock-transfected or KOR transfected HEK293 lysates onto a 7.5% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane, blocked and probed with a KOR polyclonal antibody (Product # PA3-028) at a dilution of 1:5000, overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody. Denatured KOR was detected at ~60kDa. Chemiluminescent detection was performed using ECL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of KOR was performed by loading equal amounts of wheat germ lectin agarose bead enriched KOR receptor fractions from mock-transfected or KOR transfected HEK293 lysates onto a 7.5% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane, blocked and probed with a KOR polyclonal antibody (Product # PA3-028) at a dilution of 1:5000, overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody. Denatured KOR was detected at ~60kDa. Chemiluminescent detection was performed using ECL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of KOR was performed on human brain tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a KOR polyclonal antibody (Product # PA3-028) at a dilution of 1:3000, overnight at 4C in a humidified chamber. Tissues were washed, and detection was performed using an ABC kit composed of biotinylated goat anti-rabbit IgG, peroxidase-conjugated avidin, and 3-amino-9-ethylcarbazole (AEC) substrate in acetate buffer. Tissues were counterstained with hematoxylin and dehydrated to prep for mounting.