DM1232

antibody from OriGene

Targeting: CCR4 CC-CKR-4, CD194, ChemR13, CKR4, CMKBR4, k5-5

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

DM1232

Provider

OriGene

Product name

CCR4 mouse monoclonal antibody, clone KH-4F5, Purified

Antibody type

Monoclonal

Description

CCR4 mouse monoclonal antibody, clone KH-4F5, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

CCR4

Isotype

IgG

Antibody clone number

KH-4F5

Vial size

100 µg

Concentration

2.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 4: SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: Molecular weight marker, Lane 2: 2 ug of purified KH-4F5 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 2: Spectral Confocal Microscopy of CHO cells using KH-4F5 antibody . CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Figure 1: FACS analysis of BOSC23 cells using KH-4F5 antibody . BOSC23 cells were transiently transfected with an expression vector encoding either CCR4 (Red curve) or an irrelevant protein (control transfectant). Binding of KH-4F5 was detected with a PE conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.

Validation comment

FC