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LearnABIN179723
antibody from antibodies-online
Targeting: CCR4
CC-CKR-4, CD194, ChemR13, CKR4, CMKBR4, k5-5
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- ABIN179723 - Provider product page
- Provider
- antibodies-online
- Proper citation
- Antibodies-Online Cat#ABIN179723, RRID:AB_10953433
- Product name
- anti-Chemokine (C-C Motif) Receptor 4 (CCR4) antibody
- Antibody type
- Monoclonal
- Antigen
- immunisation with human CCR4 peptide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- KH-4F5
- Vial size
- 100 ug
- Concentration
- 2 mg/ml
- Storage
- Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing)
Submitted references Antagonists of CCR4 as immunomodulatory agents.
C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential.
Chemokine receptors and their role in inflammation and infectious diseases.
Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4.
The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4.
Purandare AV, Somerville JE
Current topics in medicinal chemistry 2006;6(13):1335-44
Current topics in medicinal chemistry 2006;6(13):1335-44
C-C chemokine receptor 4 expression defines a major subset of circulating nonintestinal memory T cells of both Th1 and Th2 potential.
Andrew DP, Ruffing N, Kim CH, Miao W, Heath H, Li Y, Murphy K, Campbell JJ, Butcher EC, Wu L
Journal of immunology (Baltimore, Md. : 1950) 2001 Jan 1;166(1):103-11
Journal of immunology (Baltimore, Md. : 1950) 2001 Jan 1;166(1):103-11
Chemokine receptors and their role in inflammation and infectious diseases.
Murdoch C, Finn A
Blood 2000 May 15;95(10):3032-43
Blood 2000 May 15;95(10):3032-43
Macrophage-derived chemokine is a functional ligand for the CC chemokine receptor 4.
Imai T, Chantry D, Raport CJ, Wood CL, Nishimura M, Godiska R, Yoshie O, Gray PW
The Journal of biological chemistry 1998 Jan 16;273(3):1764-8
The Journal of biological chemistry 1998 Jan 16;273(3):1764-8
The T cell-directed CC chemokine TARC is a highly specific biological ligand for CC chemokine receptor 4.
Imai T, Baba M, Nishimura M, Kakizaki M, Takagi S, Yoshie O
The Journal of biological chemistry 1997 Jun 6;272(23):15036-42
The Journal of biological chemistry 1997 Jun 6;272(23):15036-42
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified KH-4F5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified KH-4F5 antibody. Proteins were separated by SDS-PAGE and stained with RAPID StainTM Reagent.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Spectral Confocal Microscopy of CHO cells using KH-4F5. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of KH-4F5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS analysis of BOSC23 cells using KH- 4F5. BOSC23 cells were transiently transfected with an expression vector encoding either CCR4 (red curve) or an irrelevant protein (control transfectant). Binding of KH-4F5 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with CCR4 transfected cells.
