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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- AM32707PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti SOX17
- Antibody type
- Monoclonal
- Antigen
- Recombinant SOX17 Mouse protein.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BC24-3.5CH
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blotting with SOX17 Antibody Cat.-No AM32707PU-N (Clone BC24-3.5CH) used at 2 µg/ml in Blotto on SOX17 MDCK cell lysate loaded at 20ug/lane. Secondary antibody: Peroxidase conjugated rabbit anti-mouse Ig used at 1/20000 dilution in Blotto. Developed by enhanced chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- SOX17 Antibody Cat.-No AM32707PU-N staining of E11.5 SOX17 (GFP/+) heterozygous mouse embryo. SOX17 GFP is expressed in endothelial cells and gut endoderm (A & B). 3.5CH anti-SOX17 antibody staining is shown (C & D). TRITC labeled anti mouse Ig secondary antibody. The merged images (E & F) show co-localization of GFP SOX17 protein and anti SOX17 Antibody. A: GFPB: GFPC: 3.5CH anti SOX17 Antibody Cat.-No AM32707PU-ND: 3.5CH anti SOX17 Antibody Cat.-No AM32707PU-NE: MERGEF: MERGE
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- SOX17 Antibody Cat.-No AM32707PU-N (Clone BC24-3.5CH) staining of Rh18 and Rh30 Cells after treatment with Aphidicolin which blocks in Go (Upper panels) or Nocadazole which blocks in the G2M of the cell cycle (bottom panels). PE anti-mouse Ig labeled secondary antibody.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- TF1 hematopoietic stem cell line stained with 1.11FM and 3.5CH anti-SOX17 antibodies. The staining is contrasted with staining for Oct 4 (nuclear) and CD34 (cell surface/membrane ) staining. TRITC labeled anti-mouse Ig secondary antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- SOX17 Antibody Cat.-No AM32707PU-N staining of E11.5 SOX17 (GFP/+) heterozygous mouse embryo. A: low, B & C: high magnification. SOX17 expression in gut endoderm and endothelial cells is reflected by the strong staining of these cells. 3.5CH is a mouse monoclonal antibody. The background staining reflects reaction of the secondary anti-mouse Ig antibody with mouse serum Ig. Peroxidase labeled anti-mouse Ig secondary antibody, DAB substrate.
