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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- LS-C348727 - Provider product page
- Provider
- LSBio
- Product name
- Anti-SOX17 Antibody (clone BC24-3.5CH) LS-C348727
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BC24-3.5CH
- Vial size
- 100µg
- Storage
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blotting with SOX17 Antibody (Clone BC24-3.5CH) used at 2 ug/ml in Blotto on SOX17 MDCK cell lysate loaded at 20ug/lane. Secondary antibody: Peroxidase conjugated rabbit anti-mouse Ig used at 1:20000 dilution in Blotto. Developed by enhanced chemiluminescence.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- SOX17 Antibody staining of E11.5 SOX17 (GFP/+) heterozygous mouse embryo. A: low, B & C: high magnification. SOX17 expression in gut endoderm and endothelial cells is reflected by the strong staining of these cells. 3.5CH is a mouse monoclonal antibody. The background staining reflects reaction of the secondary anti-mouse Ig antibody with mouse serum Ig. Peroxidase labeled anti-mouse Ig secondary antibody, DAB substrate.
