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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA500044 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500044, RRID:AB_1528399
- Product name
- Sox17 mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)
- Antibody type
- Monoclonal
- Description
- Sox17 mouse monoclonal antibody, clone OTI3B10 (formerly 3B10)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SOX17
- Isotype
- IgG
- Antibody clone number
- OTI3B10
- Vial size
- 100 µl
- Concentration
- NULL
Submitted references Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.
Takeuchi H, Nakatsuji N, Suemori H
Scientific reports 2014 Mar 27;4:4488
Scientific reports 2014 Mar 27;4:4488
Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.
Zhang X, Cruz FD, Terry M, Remotti F, Matushansky I
Oncogene 2013 May 2;32(18):2249-60, 2260.e1-21
Oncogene 2013 May 2;32(18):2249-60, 2260.e1-21
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-SOX17 monoclonal antibody at 1:200 dilution.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HT29 cells using anti-SOX17 mouse monoclonal antibody (TA500044).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HepG2 cells using anti-SOX17 mouse monoclonal antibody (TA500044).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SOX17 mouse monoclonal antibody (TA500044) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17(RC220888).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Immunofluorescence of SOX17 protein level by using anti-SOX17 antibody in human KhES-3 cells.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SOX17 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500044)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC220888 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SOX17 antibody(TA500044), and then analyzed by flow cytometry.
- Validation comment
- FC
