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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- TA500096 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500096, RRID:AB_2255344
- Product name
- Sox17 mouse monoclonal antibody, clone OTI2G8 (formerly 2G8)
- Antibody type
- Monoclonal
- Description
- Sox17 mouse monoclonal antibody, clone OTI2G8 (formerly 2G8)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SOX17
- Isotype
- IgG
- Antibody clone number
- OTI2G8
- Vial size
- 100 µl
- Concentration
- 1.7 mg/ml
Submitted references Primary mediastinal seminomas: a comprehensive immunohistochemical study with a focus on novel markers.
MAGEC2 is a sensitive and novel marker for seminoma: a tissue microarray analysis of 325 testicular germ cell tumors.
MAGEC2 is a sensitive and novel marker for seminoma: a tissue microarray analysis of 325 testicular germ cell tumors.
Weissferdt A, Rodriguez-Canales J, Liu H, Fujimoto J, Wistuba II, Moran CA
Human pathology 2015 Mar;46(3):376-83
Human pathology 2015 Mar;46(3):376-83
MAGEC2 is a sensitive and novel marker for seminoma: a tissue microarray analysis of 325 testicular germ cell tumors.
Bode PK, Barghorn A, Fritzsche FR, Riener MO, Kristiansen G, Knuth A, Moch H
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Jun;24(6):829-35
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Jun;24(6):829-35
MAGEC2 is a sensitive and novel marker for seminoma: a tissue microarray analysis of 325 testicular germ cell tumors.
Bode PK, Barghorn A, Fritzsche FR, Riener MO, Kristiansen G, Knuth A, Moch H
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Jun;24(6):829-35
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Jun;24(6):829-35
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SOX17 (RC220888, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SOX17.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SOX17 monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-SOX17 mouse monoclonal antibody (TA500096) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SOX17(RC220888).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of A549 cells using anti-SOX17 mouse monoclonal antibody (TA500096).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Figure from citation: Corresponding conventional and immunhistochemical stains of human seminoma and embryonal carcinoma by using anti-SOX17 antibody. Seminoma: positivity for SOX17 (Left); Embryonal carcinoma: negative staining for SOX17 (Right). Dilution: 1:2000
- Validation comment
- IHC
