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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- IC1924A - Provider product page
- Provider
- R&D Systems
- Product name
- Human SOX17 APC-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SOX17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX2, rhSOX6, and rhSOX15 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Red dye
- Antigen sequence
Q9H6I2- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm.
DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation.
Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages.
Lu J, Baccei A, Lummertz da Rocha E, Guillermier C, McManus S, Finney LA, Zhang C, Steinhauser ML, Li H, Lerou PH
Stem cell research 2018 Apr;28:48-55
Stem cell research 2018 Apr;28:48-55
DMSO efficiently down regulates pluripotency genes in human embryonic stem cells during definitive endoderm derivation and increases the proficiency of hepatic differentiation.
Czysz K, Minger S, Thomas N
PloS one 2015;10(2):e0117689
PloS one 2015;10(2):e0117689
Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells.
Wong AP, Chin S, Xia S, Garner J, Bear CE, Rossant J
Nature protocols 2015 Mar;10(3):363-81
Nature protocols 2015 Mar;10(3):363-81
Endodermal differentiation of human pluripotent stem cells to insulin-producing cells in 3D culture.
Takeuchi H, Nakatsuji N, Suemori H
Scientific reports 2014 Mar 27;4:4488
Scientific reports 2014 Mar 27;4:4488
BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages.
Bernardo AS, Faial T, Gardner L, Niakan KK, Ortmann D, Senner CE, Callery EM, Trotter MW, Hemberger M, Smith JC, Bardwell L, Moffett A, Pedersen RA
Cell stem cell 2011 Aug 5;9(2):144-55
Cell stem cell 2011 Aug 5;9(2):144-55
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of SOX17 in Definitive Endoderm-differentiated BG01V Human Stem Cells by Flow Cytometry. Definitive endoderm-differentiated (Catalog # SC019) BG01V human embryonic stem cells were stained with Goat Anti-Human SOX17 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC1924A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
