600-401-938

antibody from Rockland Immunochemicals, Inc.

Targeting: MYL9 LC20, MLC2, MRLC1, MYRL2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-938

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-938, RRID:AB_828456

Product name

Anti-Myosin (RABBIT) Antibody - 600-401-938

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Tyrosine Phosphorylation of the Myosin Regulatory Light Chain Controls Non-muscle Myosin II Assembly and Function in Migrating Cells.

Aguilar-Cuenca R, Llorente-González C, Chapman JR, Talayero VC, Garrido-Casado M, Delgado-Arévalo C, Millán-Salanova M, Shabanowitz J, Hunt DF, Sellers JR, Heissler SM, Vicente-Manzanares M
Current biology : CB 2020 Jul 6;30(13):2446-2458.e6

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Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells.

Deng JT, Bhaidani S, Sutherland C, MacDonald JA, Walsh MP
PloS one 2019;14(12):e0226406

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Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function.

Lahey KA, Ronaghan NJ, Shang J, Dion SP, Désilets A, Leduc R, MacNaughton WK
PloS one 2017;12(7):e0180259

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Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration.

Logue JS, Cartagena-Rivera AX, Baird MA, Davidson MW, Chadwick RS, Waterman CM
eLife 2015 Jul 11;4:e08314

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's anti-RLC of Smooth and Non-muscle Myosin antibody to detect vascular myosin (rat aorta, lane 1) but not cardiac myosin (mouse heart, lane2). Each lane was loaded with 35 ?g of lysate. Arrowheads indicate the detection of both mono-phosphorylated (upper) and unphosphorylated (lower) forms of the protein. After blocking with 5% NGS and 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted in blocking buffer to 1:600 for 2 h at room temperature. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Validation comment

Western Blot