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Western blot
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX113275 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113275, RRID:AB_10731615
- Product name
- DP1 antibody [N2C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Submitted references Resources for the Comprehensive Discovery of Functional RNA Elements.
Sundararaman B, Zhan L, Blue SM, Stanton R, Elkins K, Olson S, Wei X, Van Nostrand EL, Pratt GA, Huelga SC, Smalec BM, Wang X, Hong EL, Davidson JM, Lécuyer E, Graveley BR, Yeo GW
Molecular cell 2016 Mar 17;61(6):903-13
Molecular cell 2016 Mar 17;61(6):903-13
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: Raji 10% SDS PAGE GTX113275 diluted at 1:10000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DP1 antibody immunoprecipitates DP1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 £gg whole cell lysate/extract of DP1 protein expressing 293T cells B : Control with 2.5 £gg of pre-immune rabbit IgG C : Immunoprecipitation of DP1 by 2.5 £gg of DP1 antibody (GTX106221) 10% SDS-PAGE The immunoprecipitated DP1 protein was detected by DP1 antibody (GTX106221) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
