MA5-11268

antibody from Invitrogen Antibodies

Targeting: TFDP1 DILC, Dp-1, DP1, DRTF1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-11268

Provider

Invitrogen Antibodies

Product name

DP1 Monoclonal Antibody (1DP06 (TFD10))

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-11268 targets DP-1 Transcription Factor in WB applications and shows reactivity with Human, mouse, and Rat samples.

Antibody clone number

1DP06 (TFD10)

Concentration

0.2 mg/mL

References

Submitted references

Gene amplification of the transcription factor DP1 and CTNND1 in human lung cancer.

Castillo SD, Angulo B, Suarez-Gauthier A, Melchor L, Medina PP, Sanchez-Verde L, Torres-Lanzas J, Pita G, Benitez J, Sanchez-Cespedes M
The Journal of pathology 2010 Sep;222(1):89-98

---

Transcriptional profiling of MCF7 breast cancer cells in response to 5-Fluorouracil: relationship with cell cycle changes and apoptosis, and identification of novel targets of p53.

Hernández-Vargas H, Ballestar E, Carmona-Saez P, von Kobbe C, Bañón-Rodríguez I, Esteller M, Moreno-Bueno G, Palacios J
International journal of cancer 2006 Sep 1;119(5):1164-75

---

ARF directly binds DP1: interaction with DP1 coincides with the G1 arrest function of ARF.

Datta A, Sen J, Hagen J, Korgaonkar CK, Caffrey M, Quelle DE, Hughes DE, Ackerson TJ, Costa RH, Raychaudhuri P
Molecular and cellular biology 2005 Sep;25(18):8024-36

---

Building an outcome predictor model for diffuse large B-cell lymphoma.

Sáez AI, Sáez AJ, Artiga MJ, Pérez-Rosado A, Camacho FI, Díez A, García JF, Fraga M, Bosch R, Rodríguez-Pinilla SM, Mollejo M, Romero C, Sánchez-Verde L, Pollán M, Piris MA
The American journal of pathology 2004 Feb;164(2):613-22

---

Dp1 is largely dispensable for embryonic development.

Kohn MJ, Leung SW, Criniti V, Agromayor M, Yamasaki L
Molecular and cellular biology 2004 Aug;24(16):7197-205

---

Dp1 is required for extra-embryonic development.

Kohn MJ, Bronson RT, Harlow E, Dyson NJ, Yamasaki L
Development (Cambridge, England) 2003 Apr;130(7):1295-305

---

Anchorage-independent transcription of the cyclin A gene induced by the E7 oncoprotein of human papillomavirus type 16.

Schulze A, Mannhardt B, Zerfass-Thome K, Zwerschke W, Jansen-Dürr P
Journal of virology 1998 Mar;72(3):2323-34

---

Anchorage-independent transcription of the cyclin A gene induced by the E7 oncoprotein of human papillomavirus type 16.

Schulze A, Mannhardt B, Zerfass-Thome K, Zwerschke W, Jansen-Dürr P
Journal of virology 1998 Mar;72(3):2323-34

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on nuclear enriched cell extracts of T98G (Lane 1), Hep G2 (Lane 2), A-431 (Lane 3) and Raji (Lane 4). The blots were probed with anti-DP-1 Mouse Monoclonal Antibody (Product # MA5-11268, 2µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~ 50 kDa band corresponding to DP-1 was observed across all cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with DP-1 Transcription Factor antibody using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of epithelial cells and lymphocytes.